MASP2 antibody (C-Term)

Catalog No. ABIN7602762

This Rabbit Polyclonal antibody specifically detects MASP2 in WB and FACS. It exhibits reactivity toward Human.

Quick Overview for MASP2 antibody (C-Term) (ABIN7602762)

Target: MASP2 (Mannan-Binding Lectin serine Peptidase 2 (MASP2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MASP2 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-MASP2 Antibody

Binding Specificity: C-Term

Purpose: Anti-MASP2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-MASP2 Antibody Picoband® (ABIN7602762). Tested in Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human MASP2, which shares 88% and 76% amino acid (aa) sequence identity with mouse and rat MASP2, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Stover, C. M., Schwaeble, W. J., Lynch, N. J., Thiel, S., Speicher, M. R. Assignment of the gene encoding mannan-binding lectin-associated serine protease 2 (MASP2) to human chromosome 1p36.3-p36.2 by in situ hybridization and somatic cell hybrid analysis. Cytogenet. Cell Genet. 84: 148-149, 1999. 2. Stover, C. M., Thiel, S., Thelen, M., Lynch, N. J., Vorup-Jensen, T., Jensenius, J. C., Schwaeble, W. J. Two constituents of the initiation complex of the mannan-binding lectin activation pathway of complement are encoded by a single structural gene. J. Immun. 162: 3481-3490, 1999. 3. Takahashi, M., Endo, Y., Fujita, T., Matsushita, M. A truncated form of mannose-binding lectin-associated serine protease (MASP)-2 expressed by alternative polyadenylation is a component of the lectin complement pathway. Int. Immun. 11: 859-863, 1999.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for MASP2

Target: MASP2 (Mannan-Binding Lectin serine Peptidase 2 (MASP2))

Alternative Name: MASP2

Background:

Synonyms: Mannan-binding lectin serine protease 2, MBL-associated serine protease 2, Mannose-binding protein-associated serine protease 2, MASP-2, MASP2

Tissue Specificity: Plasma.

Background: Mannan-binding lectin serine protease 2 is a protein that in humans is encoded by the MASP2 gene. This gene is mapped to 1p36.22. This gene encodes a member of the peptidase S1 family of serine proteases. The encoded preproprotein is proteolytically processed to generate A and B chains that heterodimerize to form the mature protease. This protease cleaves complement components C2 and C4 in order to generate C3 convertase in the lectin pathway of the complement system. The encoded protease also plays a role in the coagulation cascade through cleavage of prothrombin to form thrombin. Myocardial infarction and acute stroke patients exhibit reduced serum concentrations of the encoded protein. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.

Molecular Weight: 76 kDa

Gene ID: 10747

UniProt: O00187

Pathways: Complement System